LOCUS       40924_22408        6990 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGloSensor-20F cAMP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Stecha PF, McNamara B, Binkowski BF.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAY-2008) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711-5399, USA
REFERENCE   2  (bases 1 to 6990)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-MAY-2008) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..865)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    970..1018
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    1032..1123
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     enhancer        1275..1653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1654..1857
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1890..1908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1922..4027
                     /codon_start=1
                     /product="GloSensor cAMP"
                     /label=GloSensor cAMP
                     /protein_id="ACF75745.1"
                     /translation="MPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYV
                     NNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQH
                     PNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVD
                     EVPKGLTGKLDARKIREILIKAKKGSSGGSGGSGGGMYESFIESLPFLKSLEFSERLKV
                     VDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRG
                     QYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVAL
                     FGTNMDIVAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYA
                     EYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERE
                     LLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHL
                     PPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQI
                     IPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPT
                     LFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAI
                     LITPEGV"
     polyA_signal    complement(4069..4190)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4384..4741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4772..5806
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     misc_feature    5833..5881
                     /note="synthetic poly(A) signal/transcriptional pause site"
     polyA_signal    5833..5881
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     primer_bind     5948..5967
                     /label=RVprimer4
                     /note="RVprimer4"
     rep_origin      complement(6208..6795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt
       61 ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag
      121 gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa
      181 tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca
      241 tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc
      301 gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt
      361 cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa
      421 atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt
      481 cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct
      541 tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa
      601 gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag
      661 tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga
      721 gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca
      781 ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg
      841 cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga agcatttatc
      901 agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
      961 acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     1021 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     1081 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa ctggcctcaa
     1141 tattggccat tagccatatt attcattggt tatatagcat aaatcaatat tggctattgg
     1201 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     1261 tgaccgccat gttggcattg attattgact agttattaat agtaatcaat tacggggtca
     1321 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     1381 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     1441 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     1501 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     1561 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     1621 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     1681 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     1741 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     1801 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     1861 ttagtgaacc gtcagatcac tagaagcttt aatacgactc actatagggc tagcgatcgc
     1921 catgcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     1981 cactggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     2041 catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg
     2101 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     2161 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     2221 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     2281 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     2341 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     2401 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     2461 gattctcatt aaggccaaga agggctcgag cggaggttca ggcggctccg gaggaggtat
     2521 gtatgaaagc tttattgagt cactgccatt ccttaaatct ttggagtttt ctgaacgcct
     2581 gaaagtagta gatgtgatag gcaccaaagt atacaacgat ggagaacaaa tcattgctca
     2641 gggagattcg gctgattctt ttttcattgt agaatctgga gaagtgaaaa ttactatgaa
     2701 aagaaagggt aaatcagaag tggaagagaa tggtgcagta gaaatcgctc gatgctcgcg
     2761 gggacagtac tttggagagc ttgccctggt aactaacaaa cctcgagcag cttctgccca
     2821 cgccattggg actgtcaaat gtttagcaat ggatgtgcaa gcatttgaaa ggcttctggg
     2881 accttgcatg gaaattatga aaaggaacat cgctacctat gaagaacagt tagttgccct
     2941 gtttggaacg aacatggata ttgtagccaa aaacattaag aagggcccag cgccattcta
     3001 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
     3061 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
     3121 gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
     3181 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
     3241 cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
     3301 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa
     3361 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
     3421 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
     3481 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
     3541 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
     3601 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
     3661 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
     3721 cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg
     3781 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
     3841 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
     3901 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
     3961 catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca ctccagaagg
     4021 ggtttaaact ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
     4081 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4141 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4201 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     4261 aaaacctcta caaatgtggt aaaatcgata aggatccgtt tgcgtattgg gcgctcttcc
     4321 gctgatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttagctac
     4381 cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4501 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4561 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4621 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4681 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4741 aaagctcgat tcttctgaca ctagcgccac catgaagaag cccgaactca ccgctaccag
     4801 cgttgaaaaa tttctcatcg agaagttcga cagtgtgagc gacctgatgc agttgtcgga
     4861 gggcgaagag agccgagcct tcagcttcga tgtcggcgga cgcggctatg tactgcgggt
     4921 gaatagctgc gctgatggct tctacaaaga ccgctacgtg taccgccact tcgccagcgc
     4981 tgcactaccc atccccgaag tgttggacat cggcgagttc agcgagagcc tgacatactg
     5041 catcagtaga cgcgcccaag gcgttactct ccaagacctc cccgaaacag agctgcctgc
     5101 tgtgttacag cctgtcgccg aagctatgga tgctattgcc gccgccgacc tcagtcaaac
     5161 cagcggcttc ggcccattcg ggccccaagg catcggccag tacacaacct ggcgggattt
     5221 catttgcgcc attgctgatc cccatgtcta ccactggcag accgtgatgg acgacaccgt
     5281 gtccgccagc gtagctcaag ccctggacga actgatgctg tgggccgaag actgtcccga
     5341 ggtgcgccac ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg acaacggccg
     5401 catcaccgcc gtaatcgact ggtccgaagc tatgttcggg gacagtcagt acgaggtggc
     5461 caacatcttc ttctggcggc cctggctggc ttgcatggag cagcagactc gctacttcga
     5521 gcgccggcat cccgagctgg ccggcagccc tcgtctgcga gcctacatgc tgcgcatcgg
     5581 cctggatcag ctctaccaga gcctcgtgga cggcaacttc gacgatgctg cctgggctca
     5641 aggccgctgc gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca cacaaatcgc
     5701 tcgccggagc gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg acagcggcaa
     5761 ccgccggccc agtacacgac cgcgcgctaa ggaggtaggt cgagtttaag atctagaacc
     5821 ggtcatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     5881 gttcgaacta gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     5941 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     6001 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6721 caaacaaacc accgctggta gcggtggttt tttgtttgca agcagcagat tacgcgcaga
     6781 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6841 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6901 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6961 gacagcggcc gcaaatgcta aaccactgca
//
